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KMID : 0379119970250030233
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Korean Journal of Mycology
1997 Volume.25 No. 3 p.233 ~ p.237
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Transformation of Pleurotus sajor - caju by Complementation of PABA Requiring Mutant
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Abstract
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A PABA auxotroph of Pleurotus sajor-caju were transformed to prototrophy by using a plasmid containing pab 1 gene from Coprinus. The efficiencies of transformation of Pleurotus sajor-caju was five transformants per ㎍ of plasmid DNA. Southern blot analysis of DNA extracted from transformants demonstrated that plasmid DNA was integrated into the chromosomal DNA in multiple tandem copies. Progenies of heterokaryons between transformants of PABA and other auxotropic strains produced pab- progeny, which indicated that integration occurred at a site(s) other than the resident pab biosynthetic gene.
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